Genome engineering using DNA-binding proteins: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
نویسندگان
چکیده
........................................................................................................... x CHAPTER 1. GENERAL INTRODUCTION ........................................................ 1 BACKGROUND ................................................................................................ 1 METHODS FOR ASSEMBLING TALENs. ...................................................... 7 OVERALL GOAL AND RESEARCH AIMS ...................................................11 DISSERTATION ORGANIZATION ................................................................ 12 REFERENCES ................................................................................................. 14 CHAPTER 2. PREDICTING SUCCESS OF OLIGOMERIZED POOL ENGINEERING (OPEN) FOR ZINC FINGER TARGET SITE SEQUENCES .............................................................................................. 16 ABSTRACT ...................................................................................................... 16..................................................................................................... 16 Background: .................................................................................................. 16 Results: .......................................................................................................... 16 Conclusion: ................................................................................................... 17 BACKGROUND .............................................................................................. 17 RESULTS .......................................................................................................... 19 DISCUSSION ................................................................................................... 25 CONCLUSION ................................................................................................. 27 METHODS ....................................................................................................... 28 Definition of active and inactive ZFP target sites based on B2H assays ...... 28 Datasets of experimentally validated ZFP-target sites .................................. 28
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